Serial Measurements of Triglyceride in Three Pooled

Accuracy of the automated fluorometric measurement of triglyceride by the technique of Kessler and Lederer is limited by the instability of the baseline. Concentration of the alcoholic KOH reagent was modified, and the pump tube manifold altered to provide on-stream mixing of the KOH reagent, which overcame this difficulty. A relatively inexpensive fluorometer with the solid-state photomultiplier microphotometer, modified to pro. vide dual-scale sensitivity, was used to increase precision of measurements. Analytical results for 30 serum samples, taken from three pools of frozen serum, show the accuracy of the measurements to be ± 8 mg/lOU ml and the standard deviation ±9 mg/100 ml for a single determination.